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KMID : 0545120120220010118
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 1 p.118 ~ p.125
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HspA and HtpG Enhance Thermotolerance in the Cyanobacterium, Microcystis aeruginosa NIES-298
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Rhee Jae-Sung
Ki Jang-Seu
Kim Bo-Mi
Hwang Soon-Jin
Choi Ik-Young
Lee Jae-Seong
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Abstract
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Heat shock proteins (Hsps) play a key role in the cellular defense response to diverse environmental stresses. Here, the role of Hsp genes in the acquisition of thermotolerance in the cyanobacterium Microcystis aeruginosa NIES-298 was investigated. Twelve Hsp-related genes were examined to observe their modulated expression patterns at different temperatures (10, 15, 25, and 35oC) over different exposure periods. HspA and HtpG transcripts showed an up-regulation of expression at low temperatures (10 and 15oC) and high temperature (35oC), compared with the control (25oC). To examine their effects upon thermotolerance, we purified recombinant HspA and HtpG proteins. During a thermotolerance study at 54oC, the HspA-transformed bacteria showed increased thermotolerance compared with the control. HtpG also played a role in the defense response to acute heat stress within 30 min. These findings provide a better understanding of cellular protection mechanisms against heat stress in cyanobacteria.
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KEYWORD
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Cyanobacteria, Microcystis aeruginosa, heat shock protein, thermotolerance
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